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Methylation-sensitive restriction enzyme digestion followed by sequencing (MRE-seq) with a SacII diagram. A library was constructed based on MRE-seq using a methylation-sensitive restriction enzyme, SacII. As cancer grows, global DNA hypomethylation accelerates demethylation in both the regularity region and intragenic regions. In the cancer genome, demethylation occurs in differ regions, making a distinct pattern.
