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. 2023 Aug 19;6:864. doi: 10.1038/s42003-023-05241-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a IGHD segment usage in the combined repertoire of llama 1 and 2 for antibodies either derived from IGHV3-3 (blue) or IGHV3S53 (orange) germlines. b IGHJ segment usage in the combined repertoire of llama 1 and 2 for antibodies either derived from IGHV3-3 (blue) or IGHV3S53 (orange) germlines. c Llama IgG2 distribution in the combined repertoire of llama 1 and 2 for antibodies either derived from IGHV3-3 (blue) or IGHV3S53 (orange) germlines. d The histogram depicts the distribution of the most 3’ prime nucleotide position in the VH segment of antibodies derived from an IGHV3-3 (upper panel) and IGHV3S53 germline (lower panel). e Nucleotide length distribution of NP nucleotides and D-segment of antibodies either derived from IGHV3-3 (n = 10304 sequences, blue) or IGHV3S53 (n = 14451 sequences, orange) VH germlines (Wilcoxon rank test adjusted p-value 2e-16). f The histogram depicts the distribution of the most 5’ prime nucleotide position in the IGHJ4 segment of antibodies derived from IGHV3-3 (upper panel) and IGHV3S53 germlines (lower panel).