Fig. 5. Relative binding affinities of IGF2, pro-IGFs and IGF1 to form binary and ternary complexes with IGFBP3 and IGFBP3:ALS.

a Formation of binary complexes with IGBP3; b Formation of ternary complexes with IGFBP3:ALS. Data are presented as means ± S.D., relative to the binding affinity of IGF2. Significant differences between marked values determined using Ordinary one-way ANOVA (Dunnett´s multiple comparisons test), *p <  0.05, **p <  0.01.