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. 2023 Aug 19;6:863. doi: 10.1038/s42003-023-05239-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — IGF2 (a), big-IGF2(87) (b), big-IGF2(104) (c), pro-IGF2(156) (d), and gly pro-IGF2(156) (e). The data were evaluated using quantification of alkaline phosphatase (ALP) activity, normalized to cell viability presented as a fold change (F.C.) over non-induced cells (day 8), n = 2 independent experiments with 6 replicates (only gly pro-IGF2(156) with 4 replicates). Data are presented as mean ± SEM (n = 6 (or 4) per group), Ordinary one-way ANOVA (Dunnett´s multiple comparisons test), *p < 0.05, **p < 0.01, ****p < 0.0001. A.U. means arbitrary units.