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. 2023 Aug 19;13:13545. doi: 10.1038/s41598-023-40549-z

Table 2.

ANOVA analysis of Box-Behnken design for melanin production by A. pullulans AKW.

Source Degree of freedom Sum of Squares F-ratio Prob > F
Overall model 9 56.139 42.481 0.001
Error
 Lack of fit 3 0.136 0.151 0.9206
 Pure error 2 0.599
 Total error 5 0.734
Single
 Tyrosine (0.1, 0.5%, w/v) 1 0.024 0.163 0.7035
 Sucrose (3, 7%, w/v) 1 3.769 25.667 0.0039
 Time (120, 216 h) 1 17.458 118.895 0.0001
Interaction
 Tyrosine*Sucrose 1 0.007 0.050 0.8323
 Tyrosine*Time 1 0.018 0.121 0.7427
 Sucrose*Time 1 2.796 19.039 0.0073
Quadratic
 Tyrosine*Tyrosine 1 16.534 112.602 0.0001
 Sucrose*Sucrose 1 11.746 79.996 0.0003
 Time*Time 1 8.557 58.278 0.0006
Corrected total 14 56.873528
Model evaluation statistics
 Determination coefficient (R2) 0.9871
 Adjusted-R2 0.9639
 Pridected-R2 0.9382