TABLE 1.
Interaction of Dvl-1, Dvl-3, and Axin in the yeast two-hybrid systema
| Protein | β-Galactosidase activity
|
||
|---|---|---|---|
| Dvl-1-(1-82) (pBTM116HA) | Dvl-1 (pBTM116HA) | rAxin (pBTM116HA) | |
| Dvl-3 (pGAD) | + | + | + |
| rAxin (pGAD) | − | + | + |
| GSK-3β (pGAD) | − | − | + |
| β-Catenin (pGAD) | − | − | + |
| Ras (pGAD) | − | − | − |
a
L40 cells were cotransformed with pGAD- and pBTM116HA-derived plasmids, as indicated in parentheses. To assay for β-galactosidase activity, cells were streaked on permissive (histidine-containing) medium and incubated for 3 days at 30°C, and then β-galactosidase activity was measured as negative (−) or positive (+). All constructs except Dvl-1-(1-82) are full-length proteins.