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. 2023 Aug 17;11:e15790. doi: 10.7717/peerj.15790

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© 2023 Tong et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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(A) The targeting fragment contains homologous arms (H1, H2), FRT sites and resistance genes for screening; (B) the homologous arms (H1, H2) on both sides of the target gene are used to undergo homologous recombination; (C) the targeting fragment successfully displaces the target gene under the mediation of Red homologous recombination; (D) the pcp20 plasmid expresses FLP recombinase, recognizes the FRT sites and eliminates the resistance genes.