| Antibodies |
|
|
|
| SNAP25 |
Santa Cruz |
sc-376713 |
| MAP-2 |
Millipore |
AB5622 |
| BDNF |
Abcam |
ab203573 |
| CREB |
Cell Signaling |
#9197 |
| Phospho-Ser133 CREB |
Abcam |
ab32096 |
| PPARα |
Abcam |
ab2779 |
| PPARβ |
Santa Cruz |
sc-7197 |
| NR2A |
Cell Signaling |
4205S |
| PSD95 |
Abcam |
ab2723 |
| AP40/AP42 |
BioLegend |
803001 |
| Actin |
Abcam |
Ab6276 |
| Alexa Fluor 647 AffiniPure Donkey Anti-Rabbit IgG |
Jackson ImmunoResearch |
711-605-152 |
| Alexa Fluor 488 AffiniPure Donkey Anti-Rabbit IgG |
Jackson ImmunoResearch |
711-545-152 |
| Alexa Fluor® 647 AffiniPure Donkey Anti-Mouse IgG |
Jackson ImmunoResearch |
715-605-151 |
| Alexa Fluor 488 AffiniPure Donkey Anti-Mouse IgG |
Jackson ImmunoResearch |
715-545-150 |
| IRDye 800CW Donkey anti-goat |
Li-Cor |
926-32214 |
| IR dye 680LT Donkey anti-mouse IgG |
Li-Cor |
926-68022 |
|
| Bacterial and virus strains |
|
| Lenti-full-length (FL) PPARα |
(Roy et al.)11,28
|
PMID: 27748752 |
| Lenti-Y314D-PPARα |
(Roy et al.)28
|
PMID: 27748752 |
|
| Chemicals, peptides, and recombinant proteins |
|
| β-hydroxy β-methylbutyrate |
Sigma |
55453 |
| FBS |
Atlas |
EF-0500-A |
| DMEM/F12 |
ThermoFisher |
MT10092CV |
| Neurobasal medium |
ThermoFisher |
21103049 |
| B-27 supplement |
ThermoFisher |
17504044 |
| Antibiotic-antimycotic |
ThermoFisher |
15240062 |
| HEPES |
ThermoFisher |
15630106 |
| Neurobasal medium, minus phenol red |
ThermoFisher |
12348017 |
| L-Glutamine |
ThermoFisher |
A2916801 |
| Poly-D-lysine |
Millipore Sigma |
P6407-5MG |
|
| Critical commercial assays |
|
| Lanthascreen TR-FRET PPARα coactivator assay kit |
ThermoFisher |
PV4684 |
| pLenti6.3/V5™-TOPO™ TA Cloning™ Kit |
ThermoFisher |
K531520 |
| ViraPower™ Lentiviral Packaging Mix |
ThermoFisher |
K497500 |
| FD Rapid Golgistain Kit |
FD Neurotechnologies |
PK401 |
| Amyloid β 40 ELISA kit |
ThermoFisher |
KMB3481 |
| Amyloid β 42 ELISA kit |
ThermoFisher |
KMB3441 |
|
| Deposited data |
|
| Raw and analyzed data |
Figshare |
doi: 10.6084/m9.figshare.22825880
|
|
| Experimental models: Cell lines |
|
| Dissociated mouse hippocampal neurons |
This study |
N/A |
| Mouse primary astrocytes |
This study |
N/A |
|
| Experimental models: Organisms/strains |
|
| Mouse: 5XFAD |
Jackson Laboratory |
(APPwFILon, PSEN1*M146L*L286V)6799Vas/J |
| Mouse: 5XFADΔPPARα
|
(Corbett et al.)29
|
PMID: 26080426 |
| Mouse: C57BL/6 |
Envigo |
C57BL/6JOlaHsd |
| Mouse: PPARα−/−
|
Jackson Laboratoty |
B6; 129S4-Pparatm1Gonz/J
|
| Mouse: PPARβ−/−
|
(Jana et al.)49
|
PMID: 22879602 |
|
| Recombinant DNA |
|
| pCMV6-AC-GFP lentiviral backbone expressing TurboGFP |
OriGene |
PS100010 |
| Mouse PPARα ORF in pCMV6-AC-GFP vector |
OriGene |
MG 227641 |
|
| Software and algorithms |
|
| Fiji (ImageJ2) |
(Schneider et al.)50
|
PMID: 22930834 |
| Morpheus |
Broad Institute |
https://software.broadinstitute.org/morpheus/
|
| GraphPad Prism Version 9.5.1 (733) |
GraphPad Software Inc. |
https://www.graphpad.com/scientific-software/prism/
|
| EthoVision XT video tracking software |
BASLER |
21743823 |
|
| Other |
|
| Victor X2 Luminescence spectrometer |
Perkin-Elmer |
20301379 |
| Victor X5 Luminescence spectrometer |
Perkin-Elmer |
20301983 |
| Olympus fluorescent microscope |
Olympus |
BX41 |
| Odyssey Infrared Imaging System |
Li-Cor |
ODY-1180 |
