Table 2.

Primer sequences of PCR amplification and fluorescent probes in ddPCR method

Mutations	Forward primer	Reverse primer	Probe (FAM)	Probe (VIC)
m.3243 A > G	CCACACCCACCCAAGAACAG	GGGTATGTTGTTAAGAAGAGGAATTGA	TTTGTTAAGATGGCAGAGC	TTGTTAAGATGGCAGGGC
m.8993 T > G	GTTATTATCGAAACCATCAGCCT	AGTAGGTGGCCTGCAGTAATGT	CAATAGCCCTGGCC	CAATAGCCCGGGCC
m.9185 T > C	GCAATATCAACCATTAAC	TTAGTGTGTTGGTTAGTA	TAGTAAGCCTCTACCTGCA	TAGTAAGCCCCTACCTGCA
m.10191 T > C	CATAGAAAAATCCACCCCTTACGA	AGGAGGGCAATTTCTAGATCAAATAA	ACCCTATACCCCCCGCC	ACCCTATATCCCCCGC