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. 2023 Aug 7;10:1170347. doi: 10.3389/fvets.2023.1170347

Table 1.

Effect of Mito-Tempo on post-thaw sperm parameters in treatment groups.

Group A (control) Group B (5 μM) Group C (50 μM) Group D (100 μM) Overall P-value
Median IQR Median IQR Median IQR Median IQR
Motile spermatozoa (%) 33.75 25.00 27.50 11.87 30.00 23.12 30.00 16.25 0.84
Morphologically normal spermatozoa (%) 61.00 16.75 63.00 23.75 65.00 17.25 61.00 16.00 0.77
Abnormal sperm head (%) 10.50 7.50 10.50 7.50 9.00 17.00 10.00 4.50 0.91
Abnormal sperm tail (%) 15.50 20.00 15.00 18.00 17.50 14.00 17.00 15.00 0.66
Proximal cytoplasmic droplet (%) 4.00 2.25 4.50 3.25 4.00 1.75 3.00 3.25 0.10
Distal cytoplasmic droplet (%) 5.00 4.50 6.50 6.25 5.00 3.50 4.50 1.75 0.13
Plasma membrane integrity (%) 42.45 15.84 35.39 12.74 37.42 15.42 41.68 12.39 0.37
Acrosome integrity (%) 65.33a 7.75 67.90b 5.55 66.67 6.55 66.46 8.17 0.07
Mitochondrial membrane potential (%) 30.25 36.52 25.94 25.34 57.95 69.78 23.82 21.16 0.99
a, b

Indicate significant differences (P ≤ 0.05).