Skip to main content
. 2023 Aug 5;26(8):107385. doi: 10.1016/j.isci.2023.107385

Figure 3.

Figure 3

Chemogenetic inhibition of VPVglut2 neurons slightly decreases wakefulness

(A) Schematic diagram of chemogenetic inhibition of VPVglut2 neurons.

(B) The expression of hM4Di-mCherry in the VP. Scale bar = 100 μm.

(C) Left: c-Fos expression in mCherry+ neurons after vehicle or CNO injection. Scale bar = 50 μm. Right: Quantification of c-Fos+mCherry+ cells divided by the total number of mCherry+ cells in the VP after vehicle or CNO injection. n = 5 mice per group. ∗∗p < 0.01, unpaired t-test.

(D) Time course changes in wakefulness, NREM sleep, and REM sleep after administration of vehicle or CNO. n = 7 mice. ∗p < 0.05, repeated-measures ANOVA followed by paired t-test.

(E) Total time spent in Wake, NREM, and REM sleep from 21:00-23:00 after administration of vehicle or CNO. n = 7 mice. ∗p < 0.05, paired t-test.

(F and G) EEG power density (F) and power spectrum (G) from 21:00-23:00 after administration of vehicle or CNO. n = 7 mice; ∗ or black line indicates p < 0.05 by paired t-test.