KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
|
| ||
| Mouse monoclonal anti-NPAT clone 27 | Santa Cruz, BD Biosciences | sc-136007, 611344 |
| Rabbit anti-SLBP | Abcam | ab221166 |
| Rabbit anti-CASP8AP2 (FLASH) | Sigma Aldrich | HPA053573 |
| Rabbit anti-Lsm11 | Sigma Aldrich | HPA039587 |
| Rabbit anti-Eri1 | Thermo Fisher | 14592-1-AP |
| Rabbit polyclonal anti-NASP | Sigma Aldrich | HPA030520 |
| Rabbit monoclonal anti-Cyclin E1 EP435E | abcam | ab33911 |
| Rabbit monoclonal anti-Cyclin E2 EP454Y | abcam | ab40890 |
| Mouse anti-Cyclin A2 | Santa Cruz | sc-271682 |
| Rabbit polyclonal anti-phospho-NPAT T1270 | Thermo Fisher-custom antibody | N/A |
| Alexa Fluor 488 anti-mouse secondary | Thermo Fisher | A-11029 |
| Alexa Fluor 546 anti-mouse secondary | Thermo Fisher | A-11030 |
| Alexa Fluor 647 anti-mouse secondary | Thermo Fisher | A-21236 |
| Alexa Flour 546 anti-rabbit secondary | Thermo Fisher | A-11035 |
| Alexa Fluor 647 anti-rabbit secondary | Thermo Fisher | A-21245 |
|
| ||
| Chemicals, peptides, and recombinant proteins | ||
|
| ||
| PF-07104091 | ChemieTek | CT-PF0710 |
| PF-06873600 | SelleckChem | S8816 |
| Palbociclib (PD-0332991) | SelleckChem | S1116 |
| RO-3306 | SelleckChem | S7747 |
| Aphidicolin | Cell Signaling | 32774 |
| ddUTP-ATTO550 | Jena Bioscience | NU-1619-550 |
| Terminal Deoxynucleotidyl Transferase | Thermo Fisher | EP0161 |
| DharmaFECT 1 | Dharmacon | T-2001-02 |
|
| ||
| Critical commercial assays | ||
|
| ||
| ViewRNA™ ISH Cell Assay Kit | Thermo Fisher | QVC0001 |
| Click-iT™ EdU Cell Proliferation Kit for Imaging | Fisher Scientific | C10340 |
|
| ||
| Experimental models: Cell lines | ||
|
| ||
| MCF10A | ATCC | CRL-10317 |
| RPE-hTERT | ATCC | CRL-4000 |
|
| ||
| Software and algorithms | ||
|
| ||
| MATLAB R2020a | Mathworks | N/A |
| Adobe Illustrator 2022 | Adobe | N/A |
| ImageJ | Fiji | N/A |
| Tracking code | Meyer Lab | https://github.com/scappell/Cell_tracking. |
| Imaris Analysis | Oxford Instruments | N/A |
|
| ||
| Deposited data | ||
|
| ||
| Imaging Data | Mendeley Data | https://doi.org/10.17632/d2b55pmjmk.1 |
|
| ||
| Oligonucleotides | ||
|
| ||
| siNPAT | Horizon Discovery | J-019599-10-0002 |
| siFLASH | Horizon Discovery | J-012413-05-0002 |
| siLsm11 | Horizon Discovery | J-018455-17-0002 |
| siCCNE1 | Horizon Discovery | M-003213-02-0005 |
| siCCNE2 | Horizon Discovery | M-003214-02-0005 |
| siCCNA2 | Horizon Discovery | M-003205-02-0005 |
| siSLBP | Horizon Discovery | J-012286-05-0002 |
| siNASP | Horizon Discovery | J-011740-13-0002 |
| siEri1 | Horizon Discovery | J-021497-09-0002 |
| siControl | Horizon Discovery | D-001810-02-05 |
| HIST2H4A | Thermo Fisher | VA6-3174283-VC |
| NPAT | Thermo Fisher | VA6-3172751-VC |
| CASP8AP2 (FLASH) | Thermo Fisher | VA6-3175253-VC |
| SLBP | Thermo Fisher | VA6-3174137-VCP |
| See Table S1 for oligonucleotides used for nascent histone RNA FISH | Integrated DNA Technologies | N/A |
|
| ||
| Other | ||
|
| ||
| Stellaris® RNA FISH Hybridization Buffer | Biosearch Technologies | SMF-HB1-10 |
| Stellaris® RNA FISH Wash Buffer A | Biosearch Technologies | SMF-WA1-60 |
| Stellaris® RNA FISH Wash Buffer B | Biosearch Technologies | SMF-WB1-20 |
| DMEM/F-12 phenol red free | Thermo Fisher | 11039047 |