Figure 4.
CNO has no effect on operant food self-administration and reinstatement of food seeking in rats with hM4Di transduction of LHb neurons (A) Training: Mean±SEM number of 35% fat pellets earned, active and inactive lever presses during training sessions over 12 days (one 3 h session/d) for rats that were trained under a fixed-ratio-1 (FR-1) 20-sec timeout reinforcement schedule. (B) Mean±SEM number of pellets self-administered after i.p. injections of vehicle or CNO (1 or 3 mg/kg) (n=12) (C) Extinction: Mean±SEM number of presses on the previously active lever or inactive lever during the extinction phase where pellets are not available (D) Mean±SEM number of active lever presses after pretreatment with CNO (3 mg) or vehicle followed by exposure to contingent tone-light cues/no cues under extinction conditions (n=14)