FIG. 7.

The TPR domain of CHIP is necessary but not sufficient for binding with Hsp70 and Hsc70. (A) Diagram depicting the location of the TPR, charged, and UFD2-like domains within the CHIP coding region. The diagram also shows the constructs of the GST-hCHIP fusion proteins that contain one or more of these domains. (B) Binding assays were performed with 1 μg of Hsp70 or Hsc70 and 15 μg of GST or GST-hCHIP fusion proteins bound to glutathione-Sepharose 4B beads. Western blots were probed with an anti-Hsp70-Hsc70 monoclonal antibody. The protein lane contains 1 μg of the specific protein indicated at the left. Sizes are indicated in kilodaltons.