Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

[Preprint]. 2023 Aug 7:2023.08.06.552176. [Version 1] doi: 10.1101/2023.08.06.552176

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.

PMC Copyright notice

Figure 2. — In vitro lineage tracing of airway M cells. (A) Violin plot of scRNA-seq profiles showing expression of Tnfrsf11a gene (RANK) in M cells as well as a small fraction of airway basal stem and secretory cells. (B) Schematic of in vitro lineage tracing of M cells in mouse tracheal ALI epithelial cultures derived from mice expressing cell-specific CreER drivers for basal stem (p63CreER) and secretory (Scgb1a1CreER) cells and the LSL-tdTomato reporter. 4-OHT stands for 4-hydroxytamoxifen. (C) Immunofluorescence images of p63CreER::tdTomato^fl/fl and Scgb1a1CreER::tdTomato^fl/fl lineage traced epithelial cells following RANKL-mediated M cell induction. Lineage positive M cells marked with arrows showing cytoplasmic tdTomato (magenta) and nuclear SOX8 (green) staining are seen in the merged panels and orthogonal sectional views. Scale bar, 20 μm. (D) Quantitative analysis of percent lineage positive M cells. Individual circles on the graph represent lineage positive cells in single RANKL-treated ALI epithelial culture wells. Data are presented as mean ± SD of triplicate ALI epithelial cultures.