pBud-C. elegans XBP-1 cloning
xbp-1	Forward: 5’- CTGAGGTACC ATGAGCAACTATCCAAAACG-3’ Reverse: 5’- GTACAGATCTTTAATTTTGGAAGAAATTTAGGTC-3’
cloning IRE1 chimera with C. elegans linker or with human linker
C. elegans IRE1 cytosolic domains	Forward: 5’- TCTGTACAAGCAATGTGGAAGACAATGGGATCAAC-3’ Reverse: 5’-GGATGAGTACCCGAGGTTAACAC-3’
	Forward 2: 5’-GTGTTAACCTCGGGTACTCATCC-3’ Reverse 2: 5’-ATACAGAATTCTTAATCGGTGTTCGGATTTGATTTC-3’
human IRE1 linker	Forward: 5’-TCTGTACAAGACTAGTCAGCTGC-3’ Reverse: 5’-GATCTAGAGGAAATTTTCCCAACTATCACC-3’
C. elegans kinase domain	Forward: 5’-AGTCTAGATATAGTCCATCAGATATTCTTGGAAC-3’
pTight-DAF-7-mCherry cloning
human IGF2 signal peptide	Forward: 5’-TTAGCGGCCGCATGGGAATCCCAATGGGGAAGTCGATGC-3’ Reverse: 5’-TATAGGTACCAGCAATGCAGCACGAGGCGAAGG-3’
mCherry	Forward: 5’-TCATGGTACCGTGAGCAAGGGCGAGGAGGATAAC −3’ Reverse: 5’-TACTAGATCTTCCTTGTACAGCTCGTCCATGCC-3’
DAF-7 propeptide	Forward: 5’-CATGTGTACAAGAACTGCACCAACTCAGGTGTTTG-3’ Reverse: 5’-TACTAGATCTTCTGAGCAACCGCATTTCTTGGCG-3’
Site-directed mutagenesis
DAF-7 C942T	Forward: 5’- CTGAGATCGGATACTGTTGCCATCCAACCGAG −3’ Reverse: 5’- CTCGGTTGGATGGCAACAGTATCCGATCTCAG −3’
DAF-7 G914C	Forward: 5’-CCTGAGATCGGATACTCCTGCCATCCA-3’ Reverse: 5’-TGGATGGCAGGAGTATCCGATCTCAGG-3’
RT-PCR
human Xbp1	Forward: 5’-AAACAGAGTAGCAGCTCAGACTGC-3’ Reverse: 5’-TCCTTCTGGGTAGACCTCTGGGAG-3’
C. elegans xbp	Forward: 5’-CGTCGTCTACGAAGAAGAAGTCGTC-3’ Reverse: 5’-GATGATAGTTAGATACATATCCACACTG-3’
qPCR primers
Rpl19	Forward: 5’-AAAACAAGCGGATTCTCATGGA-3’ Reverse: 5’-TGCGTGCTTCCTTGGTCTTAG-3’
Blos1	Forward: 5’-CAAGGAGCTGCAGGAGAAGA-3’ Reverse: 5’-GCCTGGTTGAAGTTCTCCAC-3’
mCherry	Forward: 5’-CCCGCCGACATCCCCGACTA-3’ Reverse: 5’-GGGTCACGGTCACCACGCC-3’
Gapdh	Forward: 5’-CTGGGCTACACTGAGCAGG-3’ Reverse: 5’-AAGTGGTCGTTGAGGGCAATG-3’
C.elegans actin	Forward: 5’-ATCACCGCTCTTGCCCCATC-3’ Reverse: 5’-GGCCGGACTCGTCGTATTCTTG-3’
daf-7	Forward: 5’-CGCTACAAAGGGATCCGAAC-3’ Reverse: 5’-CCCGTCGAGCATTGCCTTG-3’
ama-1	Forward: 5’-GATCTACGATCTGTGCAAAGC-3’ Reverse: 5’-CGAGTACTCGTTCGGCGGT-3’
acs-20	Forward: 5’-TCATAGAGTTGGAGCAAGACTTG-3’ Reverse: 5’-CTGAAGGAGCATCCAT GAT ACC-3’
ins-1	Forward: 5’-AACCGCTTTCAAACGTTCCG-3’ Reverse: 5’-CGTCCTGATTGCAGCAGAATG-3’