Extended Data Figure 7. Induction of genome-wide or Cas9-induced DNA breaks on nuclear chromosomes facilitate translocations involving the micronucleated chromosome.

a) Representative images of metaphase spreads hybridized to the indicated FISH probes showing examples of Y chromosome translocations. Scale bar, 10 μm. b) Frequency of inter-chromosomal rearrangements in cells following 3d DOX/IAA treatment, exposure to IR (2 Gy) or 6-thio-dG, and G418 selection. Data represent mean ± SEM of n = 3 independent experiments from (left to right): 222, 231, and 152 metaphase spreads with Y chromosome rearrangements. c) Representative images of metaphase spreads hybridized to the indicated FISH probes showing examples of normal or rearranged Y chromosomes with the indicated translocation partner. Scale bar, 10 μm. d) Frequency of Y chromosome rearrangements with the CRISPR/Cas9-targeted chromosome. Data represent mean ± SEM of n = 3 independent experiments from (left to right): 60, 75, 78,115, 84, and 77 metaphase spreads with Y chromosome rearrangements. Statistical analyses were calculated by ordinary one-way ANOVA test with multiple comparisons for panel (b) and by unpaired Student’s t-test for panel (d). **P ≤ 0.001; ***P ≤ 0.001.