Figure 5. DHED does not change hippocampal expression of estrogen receptor mRNA.

Relative expression of mRNA of estrogen receptors: ERα (Esr1) (A), ERβ (Esr2) (B) The ratio of ERα:ERβ (C), and GPER (GPER1) (D). mRNA levels were quantified using the ΔΔCq method and reported as fold change relative to vehicle-treated (veh) females. N=8–10/group. Data are represented as mean ±SEM. One-way ANOVA with Dunnett’s post hoc test vs. vehicle. Gonadally intact, vehicle-treated age-matched reference group represented on bar graphs as mean (dark green line) ±SEM (light green shading). med=medium.