Figure 2: MC-RR-induced EHBD cholangiocyte spheroid collapse is specific for neonatal cholangiocytes.

A) Degree of MC-RR-induced neonatal EHBD cholangiocyte spheroid collapse at varying MC-RR concentrations. B) Quantification showing dose dependence of lumen collapse. Representative images of spheroids treated with 200 and 300 nM MC-RR shown. Data for 400 nM MC-RR are from the experiment reported in Fig. 1. C) Quantification of loss of polarity (decrease in apical to basal actin ratio) in the presence of 400 nM MC-RR. D) Adult EHBD cholangiocyte spheroids show no damage after treatment with 400 nM of either MC-LR or MC-RR. E) Quantification showing no difference in lumen closure after treatment of adult EHBD cholangiocytes with either MC-LR or MC-RR. All images are representative of at least 3 independent experiments, with at least 18 spheroids quantified for lumen closure for each condition. Control spheroids are vehicle treated. Red: actin, Blue: DAPI. All scale bars 50 μm.