Figure 4: MC-RR treatment damages neonatal EHBD explants.

A) Representative image showing neonatal EHBD explants cultured in a high oxygen incubator in the presence of vehicle, 400 nM MC-LR, or 400 nM MC-RR. EHBD explants were imaged using confocal microscopy; a base stack and a z-stack 20 μm above base are shown. B) 3D reconstruction of confocal images of EHBD explants treated with vehicle control and 400 nM MC-RR. C) Quantification showing significant EHBD damage in the presence of 400 nM MC-RR. The number of explants studied for each condition is shown in parentheses. Red: KRT19, Blue: DAPI. All scale bars 50 μm.