Skip to main content
[Preprint]. 2023 Aug 17:arXiv:2308.06219v2. [Version 2]

Figure 2.

Figure 2.

Acoustofluidic engineering of suspended HUVECs into functional vessel-on-a chip. A) The photo of the SSAW device and microfluidic chamber. B) The simulation of the SSAW distribution. C) Before patterning (i), the cells are randomly distributed. After patterning (ii), the cells are aligned into parallel straight-line distribution. D) The separated cells (i) self-assemble at hour 24 (ii) and formed parallel perfusable vessel tubes at hour 48 (iii). 10-um-fluorescent beads can be loaded into the vessel tube (iv). E) The geometry of vessel-on-a-chip(i). The HUVECs are labeled with 488 fluorescence-conjugated-Factin. (ii) The anastomosis structure between the parallel vessel tubes and the side tube perpendicular to them. Cell nuclei is labeled with DAPI. (iii) The vessel tube under static culture condition. (iv) The vessel tube under interstitial flow-stimulated culture condition. F) The vessel tubes are characterized with the immunostaining. Cell nuclei is labeled with DAPI (i). Vessel biomarker is labeled with red CD31 (ii). Cytoskeleton is labeled with green Factin (iii). The overlap image is presented as (iv).