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[Preprint]. 2023 Aug 10:rs.3.rs-3213777. [Version 1] doi: 10.21203/rs.3.rs-3213777/v1

Figure 1: Immunity from prior A. baumannii infection.

Figure 1:

a) 6-7 week old C57/Bl6 mice were catheterized and infected with A. baumannii strain ACICU or mock-infected with PBS. Urine was collected to monitor infection status. Mice received 10 days of oral apramycin (1 mg/L) at Week 5 to clear the infection. At week 8, mice were catheterized and challenged with A. baumannii strain ACICU and sacrificed 24 hours post infection. b) Bladder and kidney homogenates and serum from Weeks 3, 5, and 8 were assayed for Abp1DRBD- and Abp2DRBD-specific IgG by ELISA. Heatmaps were generated by calculating the area under the curve for each serum/tissue sample. c) Bladder, catheter, and kidney titers were enumerated. Dashed lines indicate limit of detection. Mann-Whitney U-test, ***P≤0.0005, **P≤0.005.