Table 3.
Kinetic parameters for all evaluated bindings with two state reaction model
| Ligand RNA | Analyte Protein | Kon1 (M−1 S−1) | Koff1 (S−1) | Kon2 (M−1 S−1) | Koff2 (S−1) | KD(nM) | Rmax (RUmax) |
|---|---|---|---|---|---|---|---|
|
| |||||||
| rNEAT2 | R471H | 2.59×104 | 2.17×10−2 | 6.02×10−3 | 4.54×10−4 | 58.8 | 6.97×102 |
| R298P | 2.93×104 | 2.28×10−2 | 3.68×10−3 | 3.11×10−3 | 356 | 3.73×102 | |
| rNEAT2-m6A | R471H | 2.73×104 | 2.22×10−2 | 5.82×10−3 | 4.85×10−4 | 62.8 | 5.03×102 |
| R298P | 2.38×104 | 2.77×10−2 | 6.09×10−3 | 5.23×10−3 | 538 | 3.23×102 | |
| r6T | R471H | 4.43×104 | 3.85×10−2 | 5.06×10−3 | 7.21×10−4 | 108 | 4.19×102 |
| R298P | 5.38×103 | 4.00×10−2 | 2.14×10−3 | 8.79×10−4 | 2160 | 5.52×102 | |
| r6T-m6A | R471H | 6.87×106 | 8.99 | 6.74×10−3 | 1.01×10−3 | 171 | 2.70×102 |
| R298P | 6.62×102 | 3.66×10−2 | 2.09×10−3 | 1.48×10−3 | 22900 | 2.47×103 | |