Figure 2. CaMKII activity in PT neurons is required for delay learning.

a. Network architecture and cell types in ALM.

b. Expression of paAIP2 in ALM PT_upper neurons.

c. The effect of paAIP2 manipulation in distinct cell types in ALM. ‘No’ and ‘ALM’ are duplicated from Figure 1c for comparison purposes. Thick lines, mean ± SEM. Thin lines, individual mice (n > 3 mice per condition). *: p < 0.05 (bootstrap followed by Bonferroni correction; Extended Data Table 3).

d. op, cell-type-specific KO of CaMKIIα using CRISPR/Cas9. Bottom, immunohistochemical validation of loss of CaMKIIα protein expression in PT_upper neurons.

e. The effect of CaMKIIα KO in PT_upper and IT neurons. Thick lines, mean ± SEM. Thin lines, individual mice (n > 4 mice per condition). *: p < 0.05 (bootstrap followed by Bonferroni correction; Extended Data Table 3)

f. The effect of inactivating Cofilin in PT_upper and IT neurons. Thick lines, mean ± SEM. Thin lines, individual mice (n > 4 per condition). *: p < 0.05 for both Cofilin-SN light on vs. off comparison and Cofilin-SN light on vs. unconjugated light on conditions (bootstrap followed by Bonferroni correction; Extended Data Table 3).