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. Author manuscript; available in PMC: 2023 Sep 8.
Published in final edited form as: Nature. 2023 Mar 8;615(7953):697–704. doi: 10.1038/s41586-023-05787-1

Extended Data Fig. 3 |. Captured neoTCR specific neoantigen–HLA binding validation.

Extended Data Fig. 3 |

After capture of the neoantigen-specific T cells, the cognate TCR is sequenced, and the sequence used to gene edit healthy donor T cells replacing the endogenous TCR by the neoTCR. The neoTCR specificity and stability are validated by multimer staining of the gene-edited T cells. Only validated TCRs are shown. a-f. Multimer staining of the gene edited T-cell products gated on live cells from patient 1 or Live/CD45+ cells from patients 2, 6, 9, 10, 11 (n = 1 for patient 1, n = 2 for patient 6, 9 and 11, and n = 3 for patients 2 and 10). Mean ± SD and individual values are plotted. (n) indicates the number of technical replicates.