Extended Data Fig. 5 |. Titration of cytokine secretion by neoantigen-specific TCRs from patient 1 upon binding to specific neoantigen–HLA multimer.

Healthy donor T cells genetically engineered to express the captured neoTCRs from patient 1 were incubated with increasing amounts of plate-bound neoantigen–HLA multimer specific for each TCR and, 24 h after incubation, the IFNγ, IL-2, and TNF secreted were measured. a, NeoTCRs targeting UBE2J1 mutation presented by HLA-A*24:02. b, NeoTCRs targeting NUP188 mutation presented by HLA-A*24:02. c, NeoTCRs targeting WDR1 mutation presented by HLA-A*03:01. d, NeoTCRs targeting NRP1 mutation presented by HLA-A*03:01. e, NeoTCRs targeting TRAPPC10 mutation presented by HLA-C*04:01. f, NeoTCRs targeting IFNLR1 mutation presented by HLA-A*03:01. g, NeoTCRs targeting PLA2G4A mutation presented by HLA-A*03:01. h, NeoTCRs targeting SLC6A3 mutation presented by HLA-A*03:01. i, NeoTCRs targeting GSDMB mutation presented by HLA-C*12:02. j, Summary EC₅₀ calculated for each TCR and each cytokine. (n = 2), (n) indicates the number of biological replicates. Mean and individual values are shown. All T-cell products contain CD8⁺ and CD4⁺ gene-edited T cells.