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. Author manuscript; available in PMC: 2024 Aug 1.
Published in final edited form as: DNA Repair (Amst). 2023 Jun 8;128:103515. doi: 10.1016/j.dnarep.2023.103515

Figure 6.

Figure 6.

Effects of N-terminus motif on UDGase activity of mUNG and MHV68UNG. Summary of specific activity on uracil in ssDNA (black bar) or G:U mismatch in dsDNA (grey bar) in UDGase assay of mUNG and MHV68UNG compared to (A) mutants with deleted amino-terminal domains (mUNGΔ, MHV68UNGΔ) or (B) chimeras with swapped amino-terminal domains (m/MHV68UNG, MHV68UNG/mUNG). Average from n=3 independent experiments. (C) Representative denaturing PAGE gels from the UDGase assay resolving indicated dosage of mUNG, MHV68UNG or mutant incubated with 333 nM G:U mismatch in dsDNA or (D) ssDNA substrate containing an uracil for the indicated time. Upper band (intact substrate) and lower band (cleaved product of UDGase activity) are resolved on the gel images. Uncropped gel images included in supplemental data.