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. 2023 Aug 9;19(8):e1011578. doi: 10.1371/journal.ppat.1011578

Fig 5. Binding of purified BbEng1 to various substrates.

Fig 5

A. Western blots probed with anti-BbEng1 antibody after pull-down assays with indicated polysaccharides and silkworm cuticles as substrates (2%, w/v). The protein concentration of BbEng1 used in assays was 60 μg / mL. B, C. TLC and HPLC analyses for the carbohydrates released from the reaction of BbEng1 (2 μg, pH 6.0, 30°C for 1 h) with the extracts of polysaccharides (1%, w/v) from B. bassiana cell wall and silkworm cuticle. AS, alkali-soluble fraction. ASDN, WSDN and AIDN, alkali-soluble, water-soluble and alkali-insoluble fractions generated by nitrous deamination of fully de-N-acetylated alkali-insoluble fraction (AI).