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gp120 binding by 5145a scFv insert SUs. 3T3 cells producing the indicated viruses and SEC-CHO cells secreting HIV-1 gp120 and gp140 were labeled with [35S]cysteine. Culture supernatants were mixed as indicated, and virus particles were separated from soluble proteins by sedimentation. Samples were analyzed by RIP with hyperimmune anti-gp70 serum or human anti-HIV-1 serum, followed by SDS-PAGE and autoradiography.
