Fig. 3.

Identification of the dominant mutations in AtNHX1 gene in the sup mutants. a Positional cloning of the sup1602 mutant gene. The sup mutant alleles were identified by sequencing the AtNHX1 gene in the sup mutants. Nucleotide changes and amino acid substitutions in the gene and protein of the sup mutants are shown. b Predicted secondary structure of AtNHX1 and the amino acid substitutions in the sup mutants. The structure was illustrated using Protter (Omasits et al. 2013). c The root lengths for the different genotypes indicating improved salinity tolerance in all genotypes carrying a sup AtNHX1 allele. HZ, heterozygote, HM, homozygote. Bars represent means, and error bars represent standard errors (n = 5). Statistical significance was computed with ANOVA and Tukey’s post-hoc HSD test (P < 0.05) and different statistical groups are represented by letters