Fig. 6.

Identification of sup lines with loss-of-function mutations in AtHKT1. a sos1 suppression phenotype of the sup mutants harboring mutations in AtHKT1 gene. b Na⁺ and K⁺ contents in the callus cells of Col-gl1, hkt1, sos1–1, sos1hkt1 and sup1123. Calli were sampled (n = 3) at one (1d) and 3 days (3d) after transferring to the agar medium containing 50 mM NaCl. Confidence intervals were computed and used to determine different statistical groups within treatments, which are denoted by the letters above each bar representing the means (P < 0.05, n = 3). c Secondary structure of AtHKT1 and the amino acid substitutions in the sup mutants. The secondary structure was illustrated using Protter (Omasits et al. 2013)