Fig. 7.

Additive suppression effects of sup athkt1 and sup AtNHX1 mutants. a Root bending assay of wild type, sos1–1, sup athkt1, sup AtNHX1, and double suppressor mutants. The double suppressor mutants were generated by genetic crosses between the athkt1 and AtNHX1 sup lines. Five-day-old seedlings grown vertically in ½ MS agar (1.2%) medium were transferred to the same medium supplemented with different concentrations of NaCl, and the plates were put upside down for root bending assay. b Quantitative measurement of the root lengths of different genotypes in response to different concentrations of NaCl. Bars represent means, and error bars represent standard errors (n ≥ 3). Statistical significance among genotypes within a treatment group was computed with ANOVA and Tukey’s post-hoc HSD test (P < 0.05) and different statistical groups are represented by letters