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. 2022 Jan 17;2(1):6. doi: 10.1007/s44154-021-00025-y

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Assay for the roles of MAPKs in hyphal growth, conidiation, and sexual reproduction. a. 4-day-old CM cultures and growth rate of the wild type (WT) and Gpmk1, Gpmk1 mgv1 double, and Gpmk1 mgv1 Fghog1 triple mutants. b. Hyphal tip growth and branching patterns of the indicated strains on 1/2 CM plates. Scale bars, 50 μm. c. Conidial morphology of the indicated strains. Scale bars, 20 μm. Percentage of conidia with ≤4 and ≥ 5 septa in the indicated strains. d. Self-mating cultures of the indicated strains were examined for perithecia and cirrhi at 7 days post-fertilization. e. Asci and ascospores from the out-crosses between the mat1–1 H1-GFP strain (female) and the wild type or triple mutant (male) at 7 dpf were examined by DIC and epifluorescence microscopy. Scale bars, 20 μm. Mean and standard deviation were estimated with data from at least three (n = 3) independent biological replicates. Different letters indicate significant differences based on ANOVA analysis followed by Duncan’s multiple range test (P = 0.05)