Fig. 2.

Defects of the gmpk1 mgv1 Fghog1 triple mutant in plant infection. a. Typical wheat heads of cultivar XiaoYan 22 drop-inoculated with conidia of the wild type and Gpmk1 mgv1 Fghog1 triple mutant were examined for FHB symptoms and measured for disease index at 14 dpi. Black dots mark the inoculated spikelets. b. Corn silks inoculated with the wild type and triple mutant were examined at 5 dpi. c. Formation of infection cushions on lemma were examined by SEM. Scale bars, 10 μm. d. Infected coleoptiles were examined for necrosis and invasive hyphae after staining with Alexa Fluor 488. Scale bars, 50 μm. e. DON production in the inoculated wheat kernels sampled at 14 dpi. Mean and standard deviation were estimated with data from at least three (n = 3) independent biological replicates. The asterisk indicates significant differences based on Student’s t-test. **P < 0.01