Fig. 3.

The gmpk1 mgv1 Fghog1 triple mutant were defective in responses to abiotic stresses. a. Cultures of the indicated strains grown on regular CM and CM medium with 200 μg/ml Congo red. b. Conidia of the indicated strains were incubated for 12 h in YEPD with or without 0.7 M NaCl. Scale bars, 50 μm. c. 4-day-old cultures of the wild type and triple mutant grown on CM with or without 0.05% H₂O₂. The inhibition rate by H₂O₂ is estimated as the percentage of reduction in colony diameters on CM with H₂O₂ in comparison with regular CM. d. Percentage of conidia germinated in YEPD medium after incubation for 6 h. e. Conidia of the wild type and Gpmk1 mgv1 Fghog1 triple mutant were incubated for 12 h in YEPD with or without 0.005% H₂O₂. Scale bars, 50 μm. Conidia were indicated by arrows. f. CM cultures of the indicated strains with or without 250 μM brassinin. The inhibition rate by brassinin on growth in the indicated strains were estimated with colony diameters measured with 4-day-old cultures. g. Percentage of conidia germinated in YEPD medium after incubation for 6 h. h. Conidia of the labelled strains incubated in YEPD with or without 250 μM brassinin for 24 h. Scale bars, 50 μm. Conidia were indicated by arrows. Mean and standard deviation were estimated with data from at least three (n = 3) independent biological replicates. Different letters indicate significant differences based on ANOVA analysis followed by Duncan’s multiple range test (P = 0.05). The asterisk indicates significant differences based on Student’s t-test. **P < 0.01