Fig. 1.

SmEb is involved in ABA responses during seed germination and cotyledon greening. a The transcription levels of SmEb in 7-day-old wild-type (Col-0) seedlings after exogenous ABA treatment for 0, 3, 6, 12, 24, 48, or 72 h. The transcript levels of TUB 8 were used as an internal control. b Schematic diagram showing the T-DNA insertions in the smeb mutants. c Quantitative RT-PCR analysis of SmEb transcript levels in Col-0, smeb-1, and smeb-2 mutant plants. d 7-day-old seedlings of Col-0, smeb-1, and smeb-2 grown in 0.5× MS medium containing 0, 0.5, and 1 μM ABA. e-g Percentages of green cotyledon of Col-0, smeb-1, and smeb-2 after seed sowing for indicated days in 0.5× MS medium with different concentrations of ABA. Error bars represent the ± SD (n = 3 biological repeats). ***P < 0.001, Student’s t-test