Fig. 5.

C5 reverses the transcriptional silencing of GFP. A Symptoms of 16-TGS N. benthamiana plants inoculated with Mock, PVX, PVX-C5, or PVX-βC1 (as positive control) under white light or UV light at 15 dpi. B RT-qPCR analysis of relative GFP expression levels in the agroinfiltrated leaf patches from (A). Three plants were analyzed for each treatment and this experiment was repeated three times with similar results. Error bars represent ± SD (n = 3), and NbActin2 was used as internal reference. Student’s t test was used to statistically analyze each group of data, and double asterisks indicate significant statistical differences (**p < 0.01) between two treatments. C Western blot assays showed the accumulation of GFP and PVX CP in the agroinfiltrated leaf patches as indicated from (A). Ponceau S staining of the large RuBisCO subunit serves as loading control