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. 2022 Apr 2;2(1):19. doi: 10.1007/s44154-022-00044-3

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — C5 is important for TYLCV infection in N. benthamiana and S. lycopersicum plants. A-B Western blot analyses of TYLCV C5 and CP protein accumulation with specific anti-C5 and anti-CP antibodies in local leaves of TYLCV- and TYLCV-mC5-inoculated plants of N. benthamiana (Nb) (A) or in cotyledons of TYLCV- and TYLCV-mC5- infected plants of S. lycopersicum (Sl) (B). Wt: uninoculated plants; Mock: inoculated plants with pCambia 2300 vector; TYLCV/TYLCV-mC5: inoculated plants with the infectious clones of TYLCV/TYLCV-mC5. Ponceau S staining of the large RuBisCO subunit serves as loading control. C Symptoms of N. benthamiana plants inoculated with Mock, TYLCV, or TYLCV-mC5 infectious clones at 10 dpi. Bar = 4 cm. D Symptoms of tomato plants inoculated with Mock, TYLCV, or TYLCV-mC5 infectious clones at 14 dpi. E Western blot analyses of TYLCV CP accumulation in systemic leaves of TYLCV- and TYLCV-mC5- infected plants from (C and D). Ponceau S staining of the large RuBisCO subunit serves as loading control. F qPCR analysis of the viral DNA accumulation of TYLCV or TYLCV-mC5 -infected plants from (C and D). Three plants were analyzed for each treatment and this experiment was repeated three times with similar results. Error bars represent ± SD (n = 3) and 25S RNA was used as the internal reference. G Symptoms of YFP-C5 transgenic N. benthamiana plants infected with TYLCV or TYLCV-mC5 at 10 dpi. Bar = 4 cm. H Western blot analyses of TYLCV CP accumulation in systemic leaves of TYLCV- and TYLCV-mC5-infected plants from (G). Ponceau S staining of the large RuBisCO subunit serves as loading control. I qPCR analysis of the viral DNA accumulation of TYLCV or TYLCV-mC5 -infected plants from (G). Three plants were analyzed for each treatment and this experiment was repeated three times with similar results. Error bars represent ± SD (n = 3). Student’s t test was used to statistically analyze each group of data, and double asterisks indicate significant statistical differences (**p < 0.01) between two treatments