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. 2023 May 26;109(8):2344–2358. doi: 10.1097/JS9.0000000000000483

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Copyright © 2023 The Author(s). Published by Wolters Kluwer Health, Inc.

This is an open access article distributed under the Creative Commons Attribution-ShareAlike License 4.0, which allows others to remix, tweak, and build upon the work, even for commercial purposes, as long as the author is credited and the new creations are licensed under the identical terms. http://creativecommons.org/licenses/by-sa/4.0/

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H&E sections showing TLS and different immune cells in TLS. (A) Representative figure of H&E slides to show TLS. (B-K) IHC results showed the different immune cells, which formed the TLS, including (B) CD4⁺ T cells, (C) CD8⁺ T cells, (D) CD20⁺ B cells, (E) CD45RO⁺ memory T cells, (F) CD21⁺ follicular dendritic cells, (G) CD11c⁺ dendritic cells, (H) CD15⁺ granulocytes, (I) CD68⁺ macrophages, (J) FOXP3⁺ Treg cells, and (K) NCR1⁺ natural killer cells. (L and M) Pie chart figure showed the distribution of various immune cells forming the TLS between LCC and RCC. (N–P) Scatter plots showed different TLS maturity status between LCC and RCC. LCC, left-sided colon cancer; RCC, right-sided colon cancer.