Fig 1. Predictions of B-cell linear e and intrinsically unstructured/disordered regions (entropy) from L. infantum rDyn-1 gene.

(A) Each point inside the red boxes represents a B-cell epitopes predicted by BepiPred defined as sequence regions with values above the threshold score of 0.6 and the numbers below indicate the prediction score obtained by each epitope. The black box indicates disordered regions predicted by IUPred with a score of 0.5. Predicted regions as B-cell linear epitopes that are associated with a high degree of structural disorder also exhibit a high antigenicity score, as observed in the graph at the center of the figure. (B) Cellulose membrane containing the 17 spots with the peptide sequences derived from the rDun-1 protein selected after bioinformatics analyses visualized by ultraviolet light.