Fig 6. Comparing the reactivity of canine sera using rKDDR-plus, rDyn-1 and K39 proteins, their derived peptides K-plus 1, K-plus 2, Dyn-1 and mixtures of these three peptides and the crude extract of L. infantum in an ELISA protocol.

Reactivity index results obtained under Leishmania complete recombinant proteins Dyn-1 and KDDR-plus, the synthetic peptides derived from each recombinant protein (Dyn-1, K-plus 1 and K-plus 2 peptides), a mixture (Mix peptides) of 0.3 μg/well of the three peptides and control antigens rK39 and crude extract against a canine serological panel composed by serum from infected dogs presenting symptoms (Sym), infected asymptomatic dogs (Asy), dogs co-infected with Leishmania and Babesia sp. (CoInfec), dogs with other infections (Babesia sp., Ehrlichia sp. and T. cruzi) (Cross) and not infected (NI). The index above each column in the plot indicates the percentage of samples that are above the cut-off.