TABLE 3.
Vector production from pCHGP-transfected 293T cells
| Plasmid construct | pCMV-Rev | Titer (IU/ml)a |
|---|---|---|
| pCHGP-1 | − | (1.4 ± 0.3) × 102 |
| + | (2.5 ± 1.1) × 102 | |
| pCHGP-2 | − | (1.0 ± 0.6) × 102 |
| + | (6.0 ± 0.6) × 105 | |
| pCHGP-3 | − | (1.1 ± 0.4) × 101 |
| + | (3.6 ± 0.2) × 102 | |
| pCHGP-4 | − | (8.4 ± 1.2) × 102 |
| + | (5.3 ± 1.7) × 104 | |
| pCMV-HIV-1 | − | (1.2 ± 0.4) × 107 |
| + | (1.1 ± 0.1) × 107 |
a
The infectious vector was generated as described in Materials and Methods. Titers were determined in HT1080 cells. The numbers are the averages from triplicate experiments ± standard deviations.