Table 1.
Cryo-EM data collection and refinement statistics
| V2-Fab–KRASG12V/HLA-A*03:01 (PDB ID 7STF) |
|
|---|---|
| Data collection | |
| EM equipment | Titan Krios |
| Voltage (kV) | 300 |
| Detector | Gatan K3 |
| Energy filter | Gatan Bioquantum |
| Pixel size (Å) | 0.844 |
| Electron dose (e−/Å2) | 60 |
| Defocus range (μm) | −0.8 to −2.0 |
| Reconstruction | |
| Software | RELION 3.1 |
| Number of used particles | 116,685 |
| Symmetry imposed | C1 |
| Overall resolution (Å) | 3.14 |
| Refinement | |
| Software | Phenix |
| Cell dimensions | |
| a, b, c (Å) | 70.05, 64.14, 145.17 |
| α, β, γ (°) | 90, 90, 90 |
| Model composition | |
| Protein residues | 817 |
| R.m.s. deviations | |
| Bonds (Å) | 0.011 |
| Angles (°) | 1.705 |
| Ramachandran (%) | |
| Favorable | 81.12 |
| Allowed | 17.76 |
| Outlier | 1.12 |
| Molprobity score | 3.46 |