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. 2023 Aug 21;14(8):537. doi: 10.1038/s41419-023-06023-4

Fig. 2. SKI-178 exerts significant anti-cancer activity in cultured prostate cancer cells.

Fig. 2

The primary human prostate cancer cells (“pCan1” and “pCan2”) (A–H), the established cell lines (PC-3 and LNCaP) (F–H), the primary human prostate epithelial cells (“pEpi”) (I and J) or the established RWPE-1 epithelial cells (I, J) were cultivated in complete medium and treated with SKI-178 (10 μM or at designated concentrations for A–E) for indicated time periods; Cell viability (CCK-8 assay, A, F and I), colony formation (B), cell death (Trypan blue staining assays, C) proliferation (nuclear EdU staining assays, D, G, and J), and cell migration (“Transwell” assays, E and H) were tested by the assays mentioned in the text. “Veh” stands for vehicle control. Values represented the mean ± SD (n = 5). *P < 0.05 versus “Veh” group. Experiments in this figure were repeated five times, with similar results achieved. Scale Bar = 100 μm.