Skip to main content
. 2023 Aug 21;14(8):537. doi: 10.1038/s41419-023-06023-4

Fig. 4. The mitochondrial functions are impaired following SKI-178 treatment in prostate cancer cells.

Fig. 4

The primary human prostate cancer cells (“pCan1”) cells were cultivated in complete medium and treated with SKI-178 (10 μM) for 24 h, mitochondrial depolarization (by analyzing JC-1 green monomers, A), ROS contents (by examining CellROX fluorescence intensity, B), and single strand DNA (ssDNA) contents (ELISA OD, C) as well as the mitochondrial complex I activity (D) and the cellular ATP contents (E) were tested. The pCan1 primary cells were pretreated for 1 h with NAC (500 μM) or ATP (2 mM), followed by SKI-178 (10 μM) stimulation for72h; Cell viability and death were tested by CCK-8 (F) and Trypan blue staining (G) assays, respectively. The primary human prostate cancer cells (“pCan2”) or established cell lines (PC-3 and LNCaP) were cultivated in complete medium and treated with SKI-178 (10 μM) for 48 h; Mitochondrial depolarization (by analyzing JC-1 green monomers, H) and ROS contents (by examining CellROX fluorescence intensity, I) were tested. “Veh” stands for vehicle control. Values represented the mean ± SD (n = 5). *P < 0.05 versus “Veh” group. #P < 0.05 versus SKI-178 only treatment (F, G). Experiments in this figure were repeated five times, with similar results achieved. Scale Bar = 100 μm.