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. Author manuscript; available in PMC: 2023 Aug 22.
Published in final edited form as: Differentiation. 2022 Sep 24;128:1–12. doi: 10.1016/j.diff.2022.09.002

Fig. 3. SMAD4-I500V binds phosphorylated R-SMADs.

Fig. 3.

(A) Immunoprecipitation of WT-SMAD4 or SMAD4-I500V with anti-FLAG and detection of phospho-SMAD1,5,9. (B) Immunoprecipitation of WT-SMAD4 or SMAD4-I500V with anti-FLAG and detection of phospho-SMAD2. FLAG-WT-SMAD4, FLAG-SMAD4-I500V, or FLAG-WT-SMAD4/FLAG-SMAD4-I500V were immunoprecipitated from cell lysates at basal, TGFβ1-treated, or BMP4-treated conditions, and blotted for phosphorylated SMAD1,5,9 or phosphorylated SMAD2.