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Immunoprecipitation of HTLV-1 Gag proteins from transfected cell lysates after pulse-chase experiments. After a 30-min pulse with radioactive medium, a chase with nonradioactive medium was performed for various amounts of time. Lanes 2, 7, and 11, no chase; lanes 3, 8, 12, 1-h chase; lanes 4, 9, and 13, 4-h chase; lanes 5, 10, and 14, 8-h chase; lane 6, 12-h chase. Wild type, wild-type Gag proteins; Prec, Gag precursor; Tax, transactivator protein.
