FIG. 4.

Quantitation of BDNF, phospho-TrkB, phospho-CREB, and CB₁ using immunohistochemistry. (A) Representative images of dentate gyrus probed for expression of selected molecules: BDNF (left), phosphorylated TrkB (Tyr816, middle left), total CREB (middle right), phospho-CREB (Ser133, right). (B) Protein expression was measured within the dentate gyrus of dorsal and ventral hippocampi of treated male mice. (C) Representative images of CB₁ receptor expression in the ventral hippocampus of mice treated with vehicle (left) versus THC (right). A one-way ANOVA with multiple comparisons and Tukey's post hoc test were used to determine differences, ****p<0.0001. Each graph represents two dentate gyrus/mouse, four mice total, n=8. Error bars represent 95% confidence interval. CB₁, cannabinoid type 1 receptor; CREB, cAMP response element-binding protein.