Table 1.
Antibodies Used in This Study
| Antibody | Immunogen | Supplier | Catalog no. | Host, monoclonal vs. polyclonal | Dilution used |
|---|---|---|---|---|---|
| Immunohistochemistry | |||||
| BDNF | Synthetic human BDNF; amino acids 151–247 | Bioss, Inc., Boston, MA | bs-4989R | Rabbit pAb | 1:1,000 |
| P-TrkB | Linear synthetic rat TrkB terminus phosphorylated at Tyr816 | EMD Millipore Corp., Burlington, MA | ABN1381 | Rabbit pAb | 1:1,000 |
| CREB | Synthetic human CREB-1; amino terminus | Cell Signaling Technologies, Danvers, MA | 9197-S | Rabbit mAb | 1:2,000 |
| P-CREB | Synthetic human CREB phosphorylated at Ser133 | Cell Signaling Technologies, Danvers, MA | 9198-S | Rabbit mAb | 1:1,000 |
| Parvalbumin | Frog muscle parvalbumin | Sigma, St. Louis, MO | P3088 | Mouse mAb | 1:2,000 |
| CB1-L15 (rat sequence; amino acids 460–473) | Purified recombinant rat CB1; amino acids 460–473 | Mackie Lab59 | Guinea pig pAb | 1:2,000 | |
| Western assays | |||||
| BDNF | Synthetic human BDNF; center region | GeneTex, Irvine, CA | GTX132621 | Rabbit pAb | 1:1,000 |
| BDNF | Synthetic human BDNF; amino acids 151–247 | Bioss, Inc., Boston, MA | bs-4989R | Rabbit pAb | 1:500 |
| TrkB | Purified recombinant mouse TrkB; amino acids 32–429 | R&D Systems, Minneapolis, MN | AF1494 | Goat pAb | 1:2,000 |
| CREB | Synthetic human CREB-1; amino terminus | Cell Signaling Technologies, Danvers, MA | 9197-S | Rabbit mAb | 1:2,000 |
| VGAT | Synthetic peptide of rat VGAT; amino acids 75–87 | Synaptic Systems, Goettingen, Germany | 131011 | Mouse mAb | 1:2,000 |
| Synaptobrevin 2 | Synthetic peptide of rat synaptobrevin 2; amino acids 2–17 | Synaptic Systems, Goettingen, Germany | 104204 | Guinea pig pAb | 1:2,000 |
| PSD95 | Purified recombinant rat postsynaptic density 95 kDa | GeneTex, Irvine, CA | GTX80682 | Mouse mAb | 1:1,000 |
| VGlut2 | Purified recombinant rat VGAT; amino acids 2–115 | Synaptic Systems, Goettingen, Germany | 131004 | Guinea pig pAb | 1:1,000 |
BDNF, brain-derived neurotrophic factor; CB1, cannabinoid type 1 receptor; CREB, cAMP response element-binding protein; TrkB, tropomyosin receptor kinase B; VGAT, vesicular GABA transporter; VGlut2, vesicular glutamate transporter 2.