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. Author manuscript; available in PMC: 2023 Aug 22.
Published in final edited form as: Biochem J. 2023 May 17;480(9):685–699. doi: 10.1042/BCJ20230020

Figure 6. Disease-associated mutations in the NPT2A internal PDZ ligand block FGF23- or PTH- inhibitable phosphate transport.

Figure 6.

Arg495 (R495) in the 494TRL496 motif was substituted by Cys (R495 C) or His (R495 H). CRISPR/Cas9 Npt2a knockout OK cells were transfected with the indicated NPT2A variants. Phosphate uptake was assayed in the presence or absence of FGF23 or PTH, as indicated. n = 12, **** P < 0.0001 PTH or FGF23 vs. control.